Features

Mechanical cell wounder

Our mechanical cell wounder is a user-friendly tool for scratching cell monolayer in 96-well plates for the cell migration assay.

8-channel design
Fit for the most commonly used 96-well culture plates

Stainless steel
Highly durable, autoclavable and can be sterilized by common disinfectants

Pin holder
Holds the wounding pin

Plastic pipette tips
Provide elasticity to ensure even contact with the cell culture well surface but without causing damage can prevent cross-contamination

 

Adjustable wounding pins
Allow even contact with the culture well surface to achieve simultaneous and even wounding

Adjustable guiding bar
Ensure a fixed wounding position in each culture well; ensure the wounder can fit different culture plates with different geometries

Operation

Calibration of pins

Step 1:
Hold the wounder (fitted with pipette tips) vertically on a flat surface (e.g., 96-well plate, Petri dish) and then release the hex screw with wrench

Step 2:
Simply tap the wounder or the pins on a sterilized surface (e.g. Petri dish, culture plate lid) until all the tips touch the flat surface

Step 3:
Lock the hex screw again

Step 4:
Check the evenness of each tip by pushing the wounder on a flat sterile surface

Cell wounding

Step 1:
Place the wounder perpendicularly at the leftmost (or vice versa) end of each well within the same column of the culture plate

Step 2:
Slide the wounder horizontally to the rightmost end
(or vice versa)

Step 3:
Repeat the same scratching for all columns

Cell imaging

Data analysis

% of wound closure = [(Areat=0hr - Areat=Δhrs) / Areat=0hr ] x 100%

Why choose our product

Conventional cell scratching

The cell scratching procedure is conventionally performed by using any sharp and small objects that is commonly found in laboratories or households. They include plastic pipette tip, syringe needle, scraper, spatula, or razor blade. Obviously, none of these tools can fit different cell culture plates manufactured by different companies. The non-flexible design of conventional cell wounder cannot ensure a perfect contact with the surface of a multi-well plate, which makes it difficult to create a smooth and even “wound” cell monolayer. Such discrepancies may affect the accuracy of image analysis as well as the consistency and repetitiveness of the experiment. Moreover, as some of these devices are made of plastic, they cannot be sterilized by autoclaving; and repeated use of the same device may result in cross contamination. Lastly, users are requested to scratch the cells in the culture well or dish one by one, such practice may limit the working efficiency and increase the experimental cost.

Conventional cell wouders:

 

 

 

  1. pipette tip
  2. syringe needle
  3. scraper
  4. spatula
  5. razor blade

Disadvantages and limitation of these non-specialized cell migration tools

  • Irregular wound
  • Damage of the well surface
  • Not user-friendly
  • Large amount of experimental materials may be needed
  • Difficult in data analysis
  • Large experimental errors

Comparison of similar cell migration tools

Brand A B C D E
Wounding shape N/A regular irregular regular irregular
Materials needed large amount
(expensive)
large amount
(expensive)
large amount
(expensive)
large amount large amount
Plate format 24-well 24-well/Dish 96-well 24-well 24-well
Well surface after wounding N/A inact inact inact inact
User-friendly
Experimental error large large large small large
Replicates variation large large large small large
Quantitation difficult difficult difficult difficult difficult
Advanced imaging setup
Commerically available

Comparison of cell culture devices

  Cell culture plate
# of well 6 12 24 96 384
Vol. of medium used per well (ml) 2 0.75 0.5 0.2 0.05

  Petri dish
Size (mm) 35 50 100
Volumn (ml) 2 4 8

Mechanical cell wounder

Our newly patented mechanical cell wounder has incorporated different designs to prevent the above technical problems. It can provide flexibility in performing cell migration/ wounding assay in 96-wells culture plate format.

Contact Us

Tel: (852) 3411-5832

Email: info@rptech.com.hk